Enzyme-Activated Prodrug-Based Smart Liposomes Specifically Enhance Tumor Hemoperfusion with Efficient Drug Delivery to Pancreatic Cancer Cells and Stellate Cells

Tumor-specific enhanced delivery of chemotherapeutics and modulators to tumor cells and activated pancreatic stellate cells (aPSCs), respectively, represents safer and more effective therapy for pancreatic cancer. Herein, a membrane type 1-matrix metalloproteinase (MT1-MMP)-cleavable spacer is used to assemble low-density cRGDfK onto thermosensitive liposomes loaded with phosphorylated calcipotriol (PCAL) and doxorubicin (DOX), yielding MR-T-PD. The liposome-linked cRGDfK prodrug on MR-T-PD surface is first activated by MT1-MMP, which is selectively expressed on tumor endothelial cells, to release cRGDfK. The free cRGDfK specifically promotes tumor angiogenesis, leading to 3.4-fold higher accumulation and a wider distribution of MR-T-PD in tumors. Furthermore, MR-T-PD rapidly releases PCAL and DOX into the interstitium under heat treatment. The released DOX enters tumor cells to induce apoptosis, whereas the PCAL prodrug is converted to CAL by alkaline phosphatase on the surface of aPSCs; CAL can then enter aPSCs to induce quiescence and promote the antitumor effect of DOX. Finally, by enhancing the exposure of DOX and CAL to tumor cells and aPSCs, respectively, in a tumor-specific manner, MR-T-PD exerts superior efficacy (a 5.9-fold decrease in tumor weight) without causing additional side effects. Overall, this prodrug-based smart liposome system represents a promising paradigm for pancreatic cancer therapy.     

考虑建模误差的小型飞行器导航制导控制器设计

由于传统小型飞行器导航制导控制器对于飞行器的飞行状况调查完善度较低,数据收集力度较小,勘查条件较少.因此,本文基于建模误差设计了一种新的小型飞行器导航制导控制器.建立控制器结构,完善数据内部操作结构,获取相关性参数,并根据动力学模型,深入分析控制器数据,整合获取的信息,综合控制器设计优化算法,实现对小型飞行器导航制导控制器流程的设计.实验结果表明,相较于传统小型飞行器导航制导控制器,本文提出的设计能够在一定程度上控制数据设计的具体流向,掌控基础数据状态,具备较高的控制器反应灵敏度,且操作消耗时间较短,能够提供更为优质的系统服务.     

开关柜小车"几"字型操作手柄的研制与应用

本文针对电力系统的变电站停送电操作过程中使用的传统开关柜小车操作手柄所遇到的问题进行了分析与研究,包括绝缘手套易黏连、摇柄易脱落等,并研制了一种开关柜小车"几"字型操作手柄.该手柄已在多处现场实践应用,效果良好,大大提升了运维操作的安全可靠性,提高了工作质量和效率,在保障电力系统安全稳定运行的同时,创造了丰厚的生产经济效益.     

Synthesis,Anticancer Activity,Structure-Activity Relationship and Mechanistic Investigations of Falcarindiol Analogues

Forty samples of optically active falcarindiol analogues are synthesized by using the easily available C2 symmetric (R)- and (S)-1,1’-binaphth-2-ol (BINOL) in combination with Ti(OⁱPr)₄, Zn powder and EtI. Their anticancer activities on Hccc-9810, HepG2, MDA-MB-231, Hela, MG-63 and H460 cells are assayed to elucidate their structure-activity relationships. These results showed that the falcarindiol analogue (3R,8S)- 2 i with the terminal double bond has the most potent anti-proliferation effect on Hccc-9810 cells with IC₅₀ value of 0.46 μM. The falcarindiol analogue (3R,8S)- 2 i can induce obvious Hccc-9810 cell apoptosis in a concentration-dependent manner by Hoechst staining and flow cytometry analysis. The proposed mechanism suggests that the falcarindiol analogue (3R,8S)- 2 i increases LDH release and MDA content, and reduces the levels of SOD activity, which lead to the accumulation of oxidative stress and induce apoptosis in Hccc-9810 cells.     

基于VR技术的虚拟智能车间实训系统设计

为解决传统实训系统在应用中存在的实际问题,开展基于VR技术的虚拟智能车间实训系统设计研究.对系统服务器及车间实训内容开发板进行选型;基于VR技术对虚拟智能车间模型进行处理,演示车间实训内容等,完成基于VR技术的虚拟智能车间实训系统设计.实验证明,本文设计的实训系统与传统实训系统相比,能够实现对实训资源的高精度分配,满足学生实训学习所需.该系统为实训者提供了一个虚拟可视化的实训基地,极大地提高了实训者的应用技能,同时也为制造业的相关领域带来变革.通过实验进一步证明,设计的实训系统与传统实训系统相比,能够实现对实训资源的高精度分配,满足学生实训学习所需.     

煤炭资源型城市转型模式研究：以徐州贾汪区为例

煤炭资源型城市为我国经济发展提供了重要的资源和能源支持,研究资源型城市转型的经验模式对调整区域经济结构、确保社会稳定和改善生态环境具有重要的实践意义。本文采用文献综述法和实证分析法,研究我国东部煤炭资源枯竭型城市转型所面临的共性难题,并以徐州贾汪区转型探索经历为例,总结城市转型的"徐州贾汪区模式",主要包括放大正向外部效应、长期坚持矿地融合、大力建设矿区社会生态系统恢复力三条路径。研究结果表明,煤炭城市转型发展的共性问题相互联系、相互影响,是一个系统性难题,必须引入系统性思维。我国东部矿区普遍人口密集,农业发达、沉陷积水是最主要的共性特征,煤炭开采产生的社会问题、经济问题、生态问题、环境问题基本相同,转型发展模式值得互鉴。     

基于情境感知工具辅助碎片化学习行为的研究

碎片化学习难以集中记录,存在无法迅速定位到自己需要的学习内容等用户体验问题.传统方法不能采集真实情境下的用户数据,无法满足研究人员的研究需求.基于此,开发一种基于用户行为的捕捉工具——CAUX(Context-Aware User Experience,CAUX),设计了具有情境感知能力的数据采集模块,自动捕捉指定App内的用户行为数据.CAUX对用户干扰性低,可以辅助研究人员捕捉典型的碎片化学习行为.对利用CAUX采集到的数据进行处理,并结合人工方法进行分析,可以发现不同情境下的用户行为和用户体验问题.     

Effect of nitriding atmosphere on the morphology of AlN nanofibers from solution blow spinning

High purity AlN fiber is a promising thermal conductive material. In this work, AlN fibers were prepared using solution blow spinning followed by nitridation under N₂ or NH₃ atmosphere. Soluble polymer, such as polyaluminoxane, and allyl-functional novolac resin were adopted as raw materials to form homogeneous distribution of Al₂O₃ and C nanoparticles within the fibers, which could inhibit the growth of alumina crystal and promote their nitridation process. The effect of nitriding atmosphere on the fiber morphology was investigated. XRD results showed that complete nitridation was achieved at 1300 °C in the NH₃ or at 1500 °C in the N₂ atmosphere. Hollowed fiber structure was observed when fiber was nitrided in N₂ at high temperature, which was caused by gaseous Al gas diffusion, and this phenomenon was eliminated in NH₃ atmosphere. The nitridation mechanisms in different atmosphere were analyzed in detail. It was demonstrated that the nitridation of Al₂O₃ fibers in the NH₃ atmosphere offered the favored AlN morphology and chemical quality. Flexible AlN fiber with O content of 0.7 wt% was achieved after nitriding in NH₃ at 1400 °C. The high quality AlN can be used in thermal conductive composite materials.     

企业内部控制与CFO行为主体独立性分析

文章分析和介绍了企业内部控制的重要意义以及CFO行为主体的独立性内涵,重点剖析了企业内部控制与CFO行为主体独立性之间的内在关联,在此基础上提出了强化CFO行为主体独立性、提高企业内部控制质量的建议,以期为企业发展战略的制定提供经验参考.     

拉索检测爬升装置轻量化设计与动力学性能分析

斜拉桥的自动化、智能化检测是今后的发展趋势,目前已研发的拉索检测机器人都存在质量较大的问题,很难得到工程实际应用.针对课题组前期研制的第一代爬升装置样机质量大、爬升速度较慢等问题,对其进行了受力分析与静应力分析,得出了优化的可能性.提出了零件板厚为优化参数,强度变化率为约束条件的尺寸优化模型,利用差分进化算法求取最优解,得到了机架的最优板厚,同时通过拓扑优化得到各零件最佳结构,以及通过受力分析对螺栓的尺寸与数量进行了优化.优化结果表明爬升装置减重3.9kg,减重比例达到了40％,同时机架的变形量大幅减小,强度满足要求.对优化后的爬升装置进行了动力学仿真分析,结果表明最大爬升角度达到了75度,爬升速度提升了2-4倍,其动力性得到极大的改善.